This mouse monoclonal antibody was generated against a ΦX174 bacteriophage-derived synthetic DNA–RNA antigen and recognizes RNA-DNA hybrids of various lengths.

Highlights:

- Useful in the detection of R-loops

- High specificity and affinity for DNA-RNA hybrids

- Does NOT cross-react with single-stranded DNA or double-stranded DNA

- Minor cross-reaction (~5-fold less) has been observed for AU-rich double-stranded RNA.

- High affinity binding shown for hybrids of 8, 10, 15, and 23 base pairs in length

DNA-RNA hybrids are a natural occurrence within eukaryotic cells, with levels of these hybrids increasing at sites with high transcriptional activity, such as during transcription initiation, repression, and elongation. Because RNA-DNA hybrids influence genomic instability, the S9.6 antibody is a useful reagent to help study the consequences of R-loops and lesions formed by these hybrids during DNA replication or other cellular processes. In addition, the S9.6 antibody is effective in recognizing RNA-DNA hybridization for microarray studies.

Product Type:	Antibody
Name:	Anti-DNA-RNA Hybrid [S9.6]
Antigen:	S9.6 ΦX174 bacteriophage-derived synthetic DNA–RNA antigen
Isotype:	Mouse IgG2a
Clonality:	Monoclonal
Clone Name:	S9.6
Reactivity:	High specificity and affinity for DNA/RNA hybrids and other A-form nucleic acid hybrids
Immunogen:	ΦX174 bacteriophage-derived synthetic DNA/RNA
Species Immunized:	BALB/c mouse
Paratope:	DYYGSRWFDY (proposed)
Purification Method:	Protein A/G
Buffer:	PBS, 0.05% (w/v) Sodium Azide
Tested Applications:	Immunoblot/Immunoprecipitation,  Chip/Chip-seq, Immunocytochemistry, Immunofluorescence, DNA-RNA Immunoprecipitation (DRIP)
Storage:	-20C (avoid repeated freeze-thaw cycles)
Shipped:	Cold packs